Measurements of canine thyrotropin by means of cathodic electrochemiluminescence
Saarinen, Netta (2024)
Saarinen, Netta
2024
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Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:amk-202405069275
https://urn.fi/URN:NBN:fi:amk-202405069275
Tiivistelmä
This thesis was made in the Research & Development department of Labmaster Ltd. The objective of this thesis was to explore the possibility of developing an assay capable of measuring canine thyrotropin concentrations on the current Labmaster LUCIA™ platform. The purpose of this thesis was to conduct a feasibility study on new biomarker and to determine the parameters for the assay.
A series of experiments were conducted to test the ability of Labmaster’s LUCIA™ analyzer and its cathodic electrochemiluminescence method to measure standard solutions diluted from recombinant cTSH (canine thyroid-stimulating hormone, also known as thyrotropin). The method and parameters were optimized to improve the sensitivity of the assay.
The feasibility study was successful, the measurement results indicate that it is possible to measure cTSH with the current Labmaster LUCIA™ platform and the antibody pair effectively identifies recombinant cTSH molecules. Each parameter change improved the sensitivity of the assay and at best the sensitivity reached a cTSH concentration of 1,0 ng/ml.
Further studies are required to test the functionality of the assay with canine whole blood samples and to determine the dilution factor for whole blood samples. As the result, the required sensitivity of the assay can be estimated, and an attempt can be made to improve the sensitivity to a suitable level.
Should the assay be considered for commercialization in the future, its sensitivity must be improved without compromising its point-of-care measurability. Healthy dogs have exceptionally low levels of TSH, and when factoring in the dilution of whole blood samples, the assay may not be sensitive enough to be used to rule out hypothyroidism. However, if the antibody pair effectively identifies native cTSH molecules, and when an appropriate dilution factor is established, the assay could be used to confirm hypothyroidism.
A series of experiments were conducted to test the ability of Labmaster’s LUCIA™ analyzer and its cathodic electrochemiluminescence method to measure standard solutions diluted from recombinant cTSH (canine thyroid-stimulating hormone, also known as thyrotropin). The method and parameters were optimized to improve the sensitivity of the assay.
The feasibility study was successful, the measurement results indicate that it is possible to measure cTSH with the current Labmaster LUCIA™ platform and the antibody pair effectively identifies recombinant cTSH molecules. Each parameter change improved the sensitivity of the assay and at best the sensitivity reached a cTSH concentration of 1,0 ng/ml.
Further studies are required to test the functionality of the assay with canine whole blood samples and to determine the dilution factor for whole blood samples. As the result, the required sensitivity of the assay can be estimated, and an attempt can be made to improve the sensitivity to a suitable level.
Should the assay be considered for commercialization in the future, its sensitivity must be improved without compromising its point-of-care measurability. Healthy dogs have exceptionally low levels of TSH, and when factoring in the dilution of whole blood samples, the assay may not be sensitive enough to be used to rule out hypothyroidism. However, if the antibody pair effectively identifies native cTSH molecules, and when an appropriate dilution factor is established, the assay could be used to confirm hypothyroidism.